Abstract
Mosquitoes act as vectors of disease in humans. One method to break the chain of transmission is to reduce contact between humans and mosquitoes by using repellents. Currently, several repellents on the market contain chemicals that are harmful to users and the environment. Some researchers have conducted studies as an alternative to replace the chemicals contained in repellents by utilising extracts from natural materials. One part of the plant that is thought to have potential as a repellent is petai peel waste (Parkia speciosa Hassk.), which contains several bioactive compounds. The purpose of this study was to determine the effectiveness of petai peel waste extract in spray formulation as a repellent for Aedes aegypti mosquitoes. Extraction was carried out using the Ultrasonic Assisted Extraction (UAE) method with 96% ethanol solvent. The 25% petai peel extract formulation showed the highest repellent activity, providing over 91% protection during the first and second hours, although its effectiveness gradually declined over time, resulting in an overall average protection of 87.94%. Based on these results, petai peel waste extract has the potential to be developed as an environmentally friendly natural repellent, while also adding value to waste utilization and supporting mosquito control programs.
References
Adnani, B. (2020). Uji Potensi Ekstrak Etanol Daun Bawang Merah (Allium cepa L) Sebagai Repellent Terhadap Nyamuk Aedes aegypti. In Skripsi. Universitas Islam Negeri Maulana Malik Ibrahim.
Aji, N., Anwari, M. T., Azzahrah, N. R., & Azizah, Z. N. (2020). Pemanfaatan Limbah Kulit Buah Rambutan Sebagai Gel Tabir Surya dan Anti Bakteri Terhadap Sthaphylococcus aureus. Journal of Pharmacopolium, 3(2).
Amalia, Riska., Ahmad, Intan., Y. S. (2025). Effectiveness and Public Perception of Synthetic and Natural-Based Mosquito Repellents Against Aedes aegypti in Indonesia. 3BIO: Journal of Biological Science, Technology and Management, 7(1), 289–299. https://doi.org/10.5614/3bio.2025.7.1.1
Arsita, M. A. M., Lestari, U., Elisma, E., & Efendi, M. E. M. (2022). Physical Properties and Anti-Mosquito Activities of Lotion Male from Palm Flower Extract ( Elaeis guineensis Jacq .). Indonesian Journal of Pharmaceutical Science and Technology, 1(1), 50–59. https://doi.org/10.24198/ijpst.v1i1.42857
Banne, Y., Maramis, R., Awitari, I., Dumanauw, J., Rindengan, E., Rumagit, B., & Sapiun, Z. (2022). Pembuatan Sediaan Spray Repelen Dari Minyak Atsiri Bunga Kamboja Putih (Plumeria alba). In Prosiding Seminar Nasional Kefarmasian Program Studi Farmasi FMIPA Universitas Sam Ratulangi, 12–16. https://ejournal.unsrat.ac.id/v3/index.php/semnasfarmasi22/article/view/44419/41558
Cronquist, A. (1981). An Integrated System of Classification of Flowering Plants. Columbia University Press, New York. https://www.abebooks.com/first-edition/Integrated-System-Classification-Flowering-Plants-Cronquist/31973390061/bd
Direktorat Pupuk dan Pestisida. (2012). Metode Standar Pengujian Efikasi Pestisida Rumah Tangga dan Pengendalian Vektor T.A. 2012. https://repository.pertanian.go.id/handle/123456789/18692
Fauziah, R. R. (2017). Perbedaan Berbagai Konsentrasi Ekstrak Biji Sirsak ( Annona muricata ) sebagai Repellent terhadap Daya Tolak Nyamuk Aedes aegypti. Jurnal Riset Kesehatan, 9(2), 34–40.
Handayani, R., Qamariah, N., & Maretania, J. (2023). Formulasi sediaan serum ekstrak etanol umbi hati tanah. Jurnal Farmasetis, 12(2), 227–236. https://doi.org/10.32583/far.v12i2.1219
Hayati, R., Sari, A., & Chairunnisa, C. (2019). Formulasi Spray Gel Ekstrak Etil Asetat Bunga Melati (Jasminum sambac (L.) Ait.) Sebagai Antijerawat. Indonesian Journal of Pharmacy and Natural Product, 2(2), 59–64. https://doi.org/10.35473/ijpnp.v2i2.256
Hidana, R. (2015). Efektivitas Ekstrak Daun Sereh (Cymbopogon nardus) Sebagai Anti-Oviposisi Terhadap nyamuk Aedes aegypti. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-Ilmu Keperawatan, Analis Kesehatan Dan Farmasi, 13(1).
Hidayah, N., Mustafa, H., & Tolistiawaty, I. (2018). Efektivitas Repelan Losion Minyak Atsiri Kulit Jeruk Bali ( Citrus maxima ( Burm .) Merr .) terhadap Aedes aegypti. Balaba J Litbang Pengendali Penyakit Bersumber Binatang Banjarnegara, 14(2), 159–168. https://doi.org/10.22435/blb.v14i2.403 Efektivitas
Inayah, S., Eta, S., Billa, Z., Churrohmah, S., Azizah, R. N., & Kiromah, N. Z. (2024). Uji Aktivitas Ekstrak Etanol dan Akuades Umbi Rumput Teki ( Cyperus rotundus L .) Sebagai Repellent Terhadap Nyamuk Aedes aegypti. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 5(2), 135–141. https://doi.org/10.31764/lf.v5i2.19174
Kelik, M., & Zuliatus, E. (2018). Uji Efektivitas Ekstrak Buah Lerak (Sapindus rarak, D.C) sebagai Repelan Anti Nyamuk Aedes aegypti. Proceeding of Biology Education, 2, 42–49. https://doi.org/10.21009/pbe.2-1.6
Kementerian Kesehatan Republik Indonesia. (2024). Rencana Aksi Kegiatan (RAK) Tahun 2022 - 2024. https://repository.badankebijakan.kemkes.go.id/id/eprint/5399/1/Rencana Aksi kegiatan KGTK 2022 - 2024.pdf
Lestari, S. W. B. (2024). Identifikasi Tumbuhan Sebagai Repellent Terhadap Nyamuk : Review. Biocaster : Jurnal Kajian Biologi, 4(1), 44–51. https://doi.org/10.36312/biocaster.v4i1.245
Mangalik, A. R., Helmidanora, R., & Sa’adah, H. (2023). Formulasi Sediaan Spray Gel Ekstrak Daun Bandotan (Ageratum Conyzoides. L) Sebagai Antinyamuk. Jurnal Riset Kefarmasian Indonesia, 5(2), 245–257. https://doi.org/10.33759/jrki.v5i2.364
Mapossa, A. B., Focke, W. W., Tewo, R. K., Androsch, R., & Kruger, T. (2021). Mosquito‐repellent controlled‐release formulations for fighting infectious diseases. Malaria Journal, 20(1), 165. https://doi.org/10.1186/s12936-021-03681-7
Pradhan, D., Suri, K. A., Pradhan, D. K., & Biswasroy, P. (2013). Golden Heart of the Nature : Piper betle L . Journal of Pharmacognosy and Phytochemistry, 1(6), 147–167.
Salsabila, E., Wulandari, F., & Rohana, E. (2024). Formulation and SPF Value Evaluation of Sunscreen Spray Gel Containing Lime Peel Extract ( Citrus aurantifolia ). Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 11(1), 42–52. https://doi.org/10.20473/jfiki.v11i12024.42-52
Sari, D. Y., Ariansyah, S., Shinta, S., & Beniardi, W. (2021). Face Tonic Formulation From Ethanol Extract of Maranta arundinacea L . With Variety of Cosolvent and Surfactant : Propylene Glycol and Polysorbate 80. Proceeding of 27th International Conference ADRI, 34–39. https://doi.org/10.26737/adri27
Şengül Demirak, M. Ş., & Canpolat, E. (2022). Plant-based bioinsecticides for mosquito control: Impact on insecticide resistance and disease transmission. Insects, 13(2), 162. https://doi.org/10.3390/insects13020162
Suari, L. G., Haq, A. D., & Rahayu, L. A. (2021). Potensi Ekstrak Bunga Kamboja (Plumeria Sp.) Dan Bunga Kluwih (Artocarpus Camansi) Sebagai Biolarvasida Nyamuk Anopheles Sp. Dalam Upaya Pencegahan Penyakit Malaria. JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia, 8(3), 137–145. https://doi.org/10.53366/jimki.v8i3.267
Suwasono, H. (2009). Uji Efikasi Repelen “X” Terhadap Nyamuk Aedes aegypti, Culex auinquefasciatus Dan Anopheles aconitus di Laboratorium. Vektora: Jurnal Vektor Dan Reservoir Penyakit, 1(2), 101–108.
Verawaty, V., & Novel, D. C. (2018). Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan. Jurnal Katalisator, 3(1), 1–6. https://doi.org/10.22216/jk.v3i1.2178
Wardani, I. G. A. A. K., Rahayu, N. P. S., & Udayani, N. N. W. (2022). Efektivitas Sediaan Spray Ekstrak Bunga Tembelekan ( Lantana camara L . ) sebagai Repellent Nyamuk Aedes Aegypti. Jurnal Ilmiah Medicamento, 8(1), 8–13. https://doi.org/10.36733/medicamento.v8i1.2405
WHO. (2023). Dengue-Global Situation. Word Health Organization. https://www.who.int/emergencies/disease-outbreak-news/item/2023-DON498
WHOPES. (2009). Guidelines for Efficacy Testing of Mosquito Repellents for Human. World Health Organization. https://www.who.int/publications/i/item/WHO-HTM-NTD-WHOPES-2009.4
Widanty, N. I., Herawati, L., & Windarso, S. E. (2014). Penggunaan Mat Serbuk Daun Kemangi (Ocimum Sanctum) dan Mat Serbuk Daun Pandan Wangi (Pandanus Amaryllifolius) Sebagai Repellent Nyamuk Aedes Sp. Sanitasi: Jurnal Kesehatan Lingkungan, 6(1), 1–10.
Yanti, N. L. M., Arpiwi, N. L., & Yulihastuti, D. A. (2020). Minyak Atsiri Daun Kemangi (Ocimum × africanum Lour.) dan Efektivitasnya Sebagai Lotion Antinyamuk terhadap Aedes aegypti (Linnaeus, 1762). Metamorfosa : Journal of Biological Sciences, 7(2), 248–258. https://doi.org/10.24843/metamorfosa.2020.v07.i02.p14
Zen, S., & Asih, T. (2017). Potensi Ekstrak Bunga Tahi Kotok (Tagetes erecta) Sebagai Repellent Terhadap Nyamuk Aedes Aegypti Yang Aman Dan Ramah Lingkungan. BIOEDUKASI (Jurnal Pendidikan Biologi), 8(2), 142–149.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyright (c) 2026 Linda Septiani, Selvi Marcellia, Muhammad Maulana, Reny Arienta Putri, Michelle Safna Andari, Terza Aflika Happy, Nurul Hidayati, Arif Setiawansyah, Endah Setyaningrum, Emantis Rosa

