Comparison of Tuberculosis Examination Using Ziehl-Neelsen Method and Molecular Rapid Test

Aan Yulianingsih Anwar; Marini Tri Putri Handayani; Erpi Nurdin; Irma Berliana,HI Lewa; Artati Artati

doi:10.33490/jkm.v9i3.848

Aan Yulianingsih Anwar Departmen of Medical Laboratory Technology, Health Polytechnic of Ternate http://orcid.org/0000-0003-1464-1151
Marini Tri Putri Handayani Departmen of Medical Laboratory Technology, Health Polytechnic of Ternate http://orcid.org/0009-0008-4224-3648
Erpi Nurdin Departmen of Medical Laboratory Technology, Health Polytechnic of Ternate http://orcid.org/0000-0002-0905-7498
Irma Berliana,HI Lewa Departmen of Medical Laboratory Technology, Health Polytechnic of Ternate http://orcid.org/0009-0003-5743-8134
Artati Artati Departmen of Medical Laboratory Technology, Health Polytechnic of Makassar http://orcid.org/0000-0002-1932-9020

DOI: https://doi.org/10.33490/jkm.v9i3.848

Abstract

Tuberculosis is an infectious disease of the respiratory tract caused by infection with the bacteria Mycobacterium tuberculosis. Fast and accurate diagnostic methods are very important for diagnosing and controlling pulmonary TB disease in Indonesia. TB examination using the Zn method is very commonly used even though it has lower sensitivity than TCM. The aim of this study was to compare the results of TB examination using BTA staining with the Molecular Rapid Test. This type of research uses analytical observational methods with a cross-sectional approach. The sample was 40 respondents taken using purposive sampling technique. The results of tuberculosis examination using the Ziehl-Neelsen (ZN) BTA staining method and the Moluker rapid test tool (TCM) method showed that the microscopic results of the Ziehl-Neelsen method of BTA smear staining showed negative results in 31 people (75%), 1 respondent (2 .5%) with scanty results and 9 respondents (22.5%) with 1+ results. The results of the TB BTA examination using the TCM method resulted in MTB Not Detected with a total of 30 people (75%), MTB Detected Medium obtained a result of 6 people (15%), MTB Detected Low obtained a result with a total of 3 people (7.5%), MTB Detected Very Low results were obtained with the number of 1 person (2.5%) and in the SPSS results using the t-test the results obtained were p value <0.05 (0.012 <0.05) which means there is a comparison of the BTA staining tuberculosis examination using the Ziehl-Ziehl method. neelsen and Molecular Rapid Tests. Based on the research results, it was found that there was a significant difference between the Ziehl-Neelsen BTA staining tuberculosis examination method and the Molecular Rapid Test Tool with a p value <0.05 (0.012 <0.05)